Tetracycline-resistant bacteria and ribosomal protection protein genes in soils from selected agricultural fields and livestock farms

Abstract Antibiotic resistance in soil environment has eminently been compared and studied between agricultural pristine soils, the role of concentrated animal feeding operations markedly recognized as one major sources antibiotic resistance. This study described tetracycline small-scale farms pursuit presenting its possible contribution to persistence environment. Results would render additional information on occurrence ribosomal protection protein (RPP) tet genes among isolated bacteria from selected soils. Four RPP were determined two different settings. Both culture molecular method used determine measure soils arable land house. revealed a significantly higher number culturable antibiotic-resistant houses than lands which was suggestive areas where there direct administration antibiotics. However, quantification gene copy numbers indicated result. Higher O house (IAH-3), while other Q W found be lands. Of total 110 bacterial isolates, frequently detected, absent any isolates. Principal component analysis O, Q, also highest abundance manure Another important highlight this similarity detected identified pathogenic bacteria. Some tetracycline-resistant isolates multidrug resistant it displayed tetracycline, erythromycin, streptomycin using disk diffusion testing.